DNA

Part:BBa_K2100037

Designed by: Wangui Mbuguiro   Group: iGEM16_MIT   (2016-10-17)


pEXPR TRE - TALER14

This construct is an expression vector containing the TRE promoter upstream of the BM3R1 gene. The promoter is structured as six tetO sites upstream of a minimal CMV promoter. The induction of doxycycline activates a tetracycline transactivator protein which in turn binds to the tetO sites to initiate transcription of the TALER14 repressor. TALER14 is an engineered repressor from TAL effectors, amino acids which have corresponding DNA target sites. When transcribed, TALER14 represses the DNA sequences found on the pTALER14 promoter with the help of the corepressor VP16-Gal4.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 518
    Illegal XbaI site found at 30
    Illegal XbaI site found at 495
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 518
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 518
    Illegal BamHI site found at 641
    Illegal XhoI site found at 584
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 518
    Illegal XbaI site found at 30
    Illegal XbaI site found at 495
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal EcoRI site found at 344
    Illegal EcoRI site found at 518
    Illegal XbaI site found at 30
    Illegal XbaI site found at 495
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 656


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Categories
Parameters
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